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Metagenomic Services |
Specifications |
Catalogue No. |
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Bacterial Identification |
Protocol works for pure cultures only 16S rDNA based molecular Identity ~1,400 bp sequence data Phylogenetic Tree and 10 nearest neighbour info |
BIS 01 |
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Bacterial Population analyses : Determination of different types of Bacteria in a consortium |
RFLP-based molecular protocol Minimum no. of bacteria present predicted |
BIS 06 |
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Identification of Bacteria in a consortium |
Studies of mixed populations within Eco-samples & slurries 16S rDNA gene-pool cloned and screened All independent clones are sequenced and ~1,500 bp sequence data provided Phylogenetic Tree and 10 nearest neighbour info for all bacteria provided Charges differ based on no. of bacteria present in sample |
BIS 02 BIS 03 BIS 04 BIS 05 |
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Fungal Identification Service |
ITS region based molecular identity Raw and aligned sequence data provided Phylogenetic Tree and 10 nearest neighbor information provided |
FIS 01 |
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Fish Identification Service |
COI region sequence-based molecular identity information provided ~500 bp sequence data, Phylogenetic Tree and 10 nearest neighbour info provided |
FIS 02 |
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Plant Identification Service |
ITS region or 18S rDNA based Molecular Identity Phylogenetic tree and 10 nearest neighbour info provided |
PIS 02 |
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Development of Recombinant Microbes: By introduction of foreign genes |
A gene or set of genes are cloned and sequence confirmed The genes / gene-set can be inserted within the target genome(another microbe) Location-specific insertion on genome is possible Choice of promoters and terminators available |
MUT 03 |
| By Knocking out specific gene(s) |
A gene or set of genes are cloned and sequence confirmed The genes / gene-set can be inserted within the target genome (another microbe) The deletion would be gene specific/site specific |
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| By random mutation of gene (s) |
A particular gene/genes within the genome can be mutated randomly First, the gene(s) are cloned, characterised and mutated randomly Mutated genes are inserted within target genome individually Stable mutants delivered |
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| Methylation Analysis by Bisulfite Sequencing |
Primer design and DNA extraction Bisulfitr conversion of unmethylated Cytosines PCR amplification and gel analysis of PCR products Subloning of PCR products or direct sequencing (ABI PrismTM 3500 xl DNA Sequencer) Sequence comparison and analysis of 5MeCpGs |
BSQ 01 |
Contact services@chromous.com or call @ 09035005734 for more details