Metagenomic Services

Metagenomic Services

Specifications

Catalogue No.

Bacterial Identification

Protocol works for pure cultures only

16S rDNA based molecular Identity

~1,400 bp sequence data

Phylogenetic Tree and 10 nearest neighbour info

BIS 01

Bacterial Population analyses : Determination of different types of Bacteria in a consortium

RFLP-based molecular protocol

Minimum no. of bacteria present predicted

BIS 06

Identification of Bacteria in a consortium 

Studies of mixed populations within Eco-samples & slurries

16S rDNA gene-pool cloned and screened

All independent clones are sequenced and ~1,500 bp sequence data provided

Phylogenetic Tree and 10 nearest neighbour info for all bacteria provided

Charges differ based on no. of bacteria present in sample

BIS 02

BIS 03

BIS 04

BIS 05

Fungal Identification Service

ITS region based  molecular identity

Raw and aligned sequence data provided

Phylogenetic Tree and 10 nearest neighbor information provided

FIS 01

Fish Identification Service

COI region sequence-based  molecular identity information provided

~500 bp sequence data, Phylogenetic Tree and 10 nearest neighbour info provided

FIS 02

Plant Identification Service

ITS region or 18S rDNA based Molecular Identity

Phylogenetic tree and 10 nearest neighbour info provided

PIS 02

Development of Recombinant Microbes: 

By introduction  of  foreign genes

A gene or set of genes are cloned and sequence confirmed

The genes / gene-set can be inserted within the target genome(another microbe)

Location-specific insertion on genome is possible

Choice of promoters and terminators available

A gene or set of genes are cloned and sequence confirmed

The genes / gene-set can be inserted within the target genome (another microbe)

The deletion would be gene specific/site specific

A particular gene/genes within the genome can be mutated randomly

First, the gene(s) are cloned, characterised and mutated randomly

Mutated genes are inserted within target genome individually

Stable mutants delivered 

Primer design and DNA extraction

Bisulfitr conversion of unmethylated Cytosines

PCR amplification and gel analysis of PCR products

Subloning of PCR products or direct sequencing (ABI Prism^TM 3500 xl DNA Sequencer)

Sequence comparison and analysis of 5MeCpGs