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Genotyping Services |
Specifications |
Catalogue No. |
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Development of Microsatellite |
Microsatellite library from for any organism 20, 30, 40 or 50 Markers are developed, Charges vary Marker specific primers designed and PCRs standardized All marker sequence data, primer-pairs and protocols delivered |
SSR 01 (20 Markers) |
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SSR 02 (30 markers) |
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SSR 03 (40 markers) |
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SSR 04 (50 markers) |
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Genotyping by SSR/ STR analysis |
Once the Microsatellite marker data is available (Service Cat. # SSR 01), genotyping can be initiated Genomic DNA isolated from individual sample STR specific PCRs are performedusing flurescent primers sample PCR amplicons are sized on a Genetic analyzer
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SSR 05 (<100 markers) |
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SSR 06 (<1,000 markers) |
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SSR 07 (>1,000 markers) |
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Fragment Analysis by GeneMapper/ GeneScan |
Fluorescent-tagged PCR products are sized on Capillary Sequencer Compatible dyes are: DS-33 (Dye set G5): FAMTM, NEDTM, VIC®, PET® and LIZ® DS-30 (Dye set D): FAMTM, HEXTM, NEDTM, and ROXTM Services include: Performing PCR amplification, multiplexing (upto 4 Markers), Adding LIZ-500, Sample denaturation, Sample Run and Sizing fragments Sizing data and Chromatogram are provided. |
STR 01 (<100 runs) |
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STR 02 (>100 and <1,000 runs) |
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STR 03 (>1,000 runs) |
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Genotyping by AFLP |
Polymorphism analyses within segregating populations, varieties, strains, etc. Complete protocol starting from gDNA isolation to selective PCRs |
AFLP 01 |
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AFLP Fragement Analysis |
Fluorescent–labeled AFLP profiles analyzed Compatible dyes are: DS-33 (Dye set G5): FAMTM, NEDTM, VIC®, PET® and LIZ® DS-30 (Dye set D): FAMTM, HEXTM, NEDTM, and ROXTM Pair-wise sizing data, phylogenetic tree and Chromatogram provided. |
AFL 01 |
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Genotyping by RAPD (Non- Fluorescent primer) |
RAPD analysis using 20-mer primer Annealing at 55ºC, thus reproducible profiles 20-30 bands on profile Polymorphism generated within closely related samples as well Primer information, Agarose gel photo, etc. provided Phylogeny analyses is difficult |
RAPD 01 |
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Genotyping by RAPD (Fluorescent primer) |
RAPD analysis using fluorescent labeled 20-mer primer Annealing at 55ºC thus reproducible profiles RAPD profile analyzed on Genetic Analyzer 50-75 bands on profile Polymorphism generated within closely related samples as well Phylogeny analyses is assured |
RAPD 02 |
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Development of ARMS Marker |
Distinguishes two similar genotypes Primer-sets pecific to a genotype developed and validated RAPD based polymorphism detected ARMS primers designed and validated |
ARMS 01 |
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Authentication of Genotype by Barcoding |
Profile developed using fluorescent labeled RAPD primer Represented genome wide variation with ~1000 loci data |
BAR 01 |
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Development of SCAR Markers |
Genotyping of closely related samples Polymorphic RAPD profiles are developed for each sample Sample specific markers/ primer-sets are designed and Genotypes are validated |
DSP 01 |
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SNP detection and data Analysis |
Sequencing-based protocol Bi-directional sequence data to confirm SNPs, charged extra SNPs marked on peak data Zygosity detected based on reference sequence data provided Primers and PCR amplification conditions provided by Scientist Primers, if required, are charged extra In case PCR fails 9upon repeated attempts), we charge |
SEQ 09
SEQ 09F |
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MicroArray & Genome Sequencing Services |
Microarray service for detection expression level changes, aCGH, ChIP on chip, Methylation profiling and miRNA profiling |
MAS 01 |
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Trnscriptome Sequencing: cDNA library preparation cDNA library preparation for transcriptome Sequencing using Illumina solexa platform, Provide fresh samples, collected on dry-ice |
TLP 01 |
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Genome Sequencing: Basic Sequencing and data analysis: Raw data/basic sequence analysis without reference genome Functional analysis |
SDA 03 |
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Genome Sequencing: Solexa Illumina Protocol Paired end (per lane) @ 72 bp read length |
SS 02 |
Contact services@chromous.com or call @ 09035005734 for more details