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Research Services |
Specifications |
Catalogue No. |
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Synthetic Gene Synthesis |
We synthesize the gene, clone and sequence verify Good clone with no mutations delivered Gene expression/ protein purification services available Cat. # GEPS 01 |
SGS 01 |
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Full-Length cDNA Cloning |
Full-length cDNA from any organism Based on partial cDNA information, consensus sequence data or homologous organism sequence data Sequence confirmed clone delivered Synthetic synthesis is also available, Cat. # SGS 01 Gene expression/ protein purification services available Cat.# GEPS 01 |
FLCS 01 |
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Development and Screening of cDNA /EST Library/ SSH Library |
cDNA library developed from all sources 1,000 positive clones selected All 1,000 clones are sequenced All cDNA clones with annotated sequence data provided Sequencing of more or fewer samples |
CDN 01 |
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Gene expression and Protein purification services |
Genes are expressed in bacterial and Pichia and Mammalian systems based systems Proteins are purified for antibody development, Crystallography, assay development, etc. Proteins are solubilized and re-folded to active form as well Milligram to Gram Scale Protein expression and purification in bacterial expression system, ~5 mg Protein expression and purification in Pichia expression system, ~5 mg Protein expression and purification in Mammalian expression system Baculovirus/ Insert cell line system |
GEPS 01 GEPS 02
GEPS 03 GEPS 04
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Solubilization of insoluble protein |
Starting from insoluble / inclusion body, proteins are extracted and solubilized for activity Different refolding protocols are tried and provided to Scientists for assays More protein is solubilized following the same protocol (that performed on assay) Biological activity not guaranteed |
SIP 01 |
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Gene Engineering Services |
Development of Deletion mutants, Chimeras, Knock-out constructs, Constructs with repeat elements, etc Development of Cloning/ Expression vectors |
GES 01 |
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Mutagenesis Services |
Site-Directed mutagenesis of a cloned gene Introduce or revert mutations or delete single to multiple bases Upto 5 mutations within 10 bp stretch are counted as single service Sequence confirmed clone delivered |
MUT 01 |
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Random mutagenesis of a cloned gene Introduce random mutations in whole gene/part of genes/ domain of interest 50 random clones delivered One clone sequenced to confirm random mutations introduced. |
MUT 02 |
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siRNA Design and Synthesis |
Designed and synthesized for different systems (Size: ~250-500 bp dsRNA, 3 Nos. per dsRNA, 50 µg) siRNA and shRNA are synthesized Site-less cloning performed for perfect transcript production, no extra bases |
SIR 01
SHR 01 |
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Drosophila siRNA |
Available for silencing all Drosophila genes individually Validated to be gene-specific Provided 10 µg enough for 10 or more experiments |
DSR 01 |
Contact services@chromous.com or call @ 09035005734 for more details