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DNA Sequencing Service |
Specifications |
Catalogue No. |
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Sequencing Purified clone/ Plasmid DNA |
Single sequencing reaction 550-650 bases data |
SEQ 01 |
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Sequencing of plasmids (unpurified) |
Send clones as stabs/ slants/ culture plates, we purify DNA We send 96-well plates with LB Agar + antibiotics, for large no. of samples Good data guaranteed for >95% samples |
SEQ 01A (<100 samples) |
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SEQ 01B (<1,000 samples) |
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SEQ 01C (>1,000 samples) |
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Sequencing Purified PCR products |
Single pass analysis of 550-650 bases data |
SEQ 02 |
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Sequencing PCR products (unpurified fragments) |
Send unpurified PCR samples in PCR vials only PCR Clean-up/ Gel elution of specific band are performed Good data guaranteed for >95% samples
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SEQ 02A (<100 samples) SEQ 02B (<1,000 samples) SEQ 02C (>1,000 samples) |
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16s rDNA/ 18s rDNA/ ITS Sequencing from Specimen |
We isolate gDNA from specimen: bacteria, fungi, plant and animals We perform PCR using in-house consensus primers and sequence Sequence data aligned and delivered Sequencing would fail for mixed cultures received, will be charged in full |
SEQ 11A |
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Genomic DNA Isolation from all Biological Samples |
Column based protocol: RNase-free, DNase-free gDNA MiniPrep, upto 5 µg MidiPrep, upto 100 µg MaxiPrep, upto 500 µg |
GDI 01 GDI 02 GDI 03 |
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PCR Standardisation |
Standardisation for amplification of Microsatellites, variable regions, homologus genes, novel genes, etc |
PCS 01 |
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PCR and Sequencing |
Perform PCR and Clean-up from Genomic DNA/ Plasmid DNA/ template DNA Primers and PCR amplification conditions provided by the Scientists Full-lenght sequencing of amplicon Data aligned and provided In case PCR fails (upon repeated attempts), we charge |
SEQ 10
SEQ 10F |
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Primer-walking (Single-Strand) |
Single Strand sequencing, charged per base Internal primers are synthesized Final data aligned and delivered |
SEQ 04 |
| Primer-walking [(AT/ GC) % >70%] Single strand | Single strand | SEQ 04A |
| Primer-walking (Double-strand) |
Double Strand sequencing, charged per base Internal primers are synthesized Final data aligned and delivered |
SEQ 05 |
| Primer-walking [(AT/ GC) % >70%] | Double strand | SEQ 05A |
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Sequencing Native bacterial plasmids |
Provide pure Plasmids Complete Circular map provided Restriction map provided |
SEQ 08 |
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SNP detection and data analysis |
Sequencing-based protocol Bi-directional sequence data to confirm SNPs, charged extra SNPs marked on peak data Zygosity detected based on reference sequence data provided Primers and PCR amplification conditions provided by Scientist Primers, if required, are charged extra In case PCR fails (upon repeated attempts), we charge |
SEQ 09
SEQ 09F |
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HLA Class I Typing |
HLA-A/B/C typing by sequencing Exon 2 and 3 amplified Genomic DNA isolation included Sequence data provided |
HLAC 1 |
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HLA Class II Typing |
HLA- DRB1/ 3/ 4/ 5/ DBP1/ DQA1/ DQB1 typing by sequencing Exon 2 and 3 amplified Genomic DNA isolation included Sequence data provided |
HLAC 2 |
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SSCP analysis |
SSCP 01 |
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| PCR, Cloning & Sequencing |
500 bp or smaller amplicons, 1- 4 samples 500 bp or smaller amplicons, >4 samples 500 bp to 1.0 kb amplicons, 1- 4 samples 500 bp to 1.0 kb amplicons, >4 samples 1.0 kb to 2.0 kb amplicons, 1- 4 samples 1.0 kb to 2.0 kb amplicons, >4 samples |
CSS 01 CSS 02 CSS 03 CSS 04 CSS 05 CSS 06 |
Contact services@chromous.com or call @ 09035005734 for more details