Our Products



DNA Sequencing Services

DNA Sequencing Service

Specifications

Catalogue No.

Sequencing Purified clone/ Plasmid DNA

Single sequencing reaction

550-650 bases data

SEQ 01

Sequencing of plasmids (unpurified)

Send  clones  as  stabs/ slants/ culture plates, we purify DNA

We send 96-well plates with LB Agar + antibiotics, for large no. of samples

Good data guaranteed for >95% samples

SEQ 01A (<100 samples)

SEQ 01B (<1,000 samples)

SEQ 01C (>1,000 samples)

Sequencing Purified PCR products

Single pass analysis of 550-650 bases data

SEQ 02

Sequencing PCR products (unpurified fragments)

 Send unpurified PCR samples in PCR vials only

PCR Clean-up/ Gel elution of specific band are performed

Good data guaranteed for >95% samples

 

SEQ 02A (<100 samples)

SEQ 02B (<1,000 samples)

SEQ 02C (>1,000 samples)

16s rDNA/ 18s rDNA/ ITS Sequencing from Specimen

We isolate gDNA from specimen: bacteria, fungi, plant and animals

We perform PCR using in-house consensus primers and sequence

Sequence data aligned and delivered

Sequencing would fail for mixed cultures received, will be charged in full

SEQ 11A

Genomic DNA Isolation from all Biological Samples

Column based protocol: RNase-free, DNase-free gDNA

MiniPrep, upto 5 µg

MidiPrep, upto 100 µg

MaxiPrep, upto 500 µg

 

GDI 01

GDI 02

GDI 03

PCR Standardisation

Standardisation for amplification of Microsatellites, variable regions, homologus genes, novel genes, etc

PCS 01

PCR and Sequencing

Perform PCR and Clean-up from Genomic DNA/ Plasmid DNA/ template DNA

Primers and PCR amplification conditions provided by the Scientists

Full-lenght sequencing of amplicon

Data aligned and provided

In case PCR fails (upon repeated attempts), we charge

 SEQ 10

 

 

 

 

SEQ 10F

Primer-walking (Single-Strand)

Single Strand sequencing, charged per base

Internal primers are synthesized

Final data aligned and delivered

SEQ 04

Primer-walking [(AT/ GC) % >70%] Single strand        Single strand   SEQ 04A
Primer-walking (Double-strand)

Double Strand sequencing, charged per base

Internal primers are synthesized

Final data aligned and delivered

SEQ 05
Primer-walking [(AT/ GC) % >70%] Double strand SEQ 05A

Sequencing Native bacterial plasmids  

Provide pure Plasmids

Complete Circular map provided

Restriction map provided

SEQ 08

SNP detection and data analysis

Sequencing-based protocol

Bi-directional sequence data to confirm SNPs, charged extra

SNPs marked on peak data Zygosity detected based on reference sequence data provided

Primers and PCR amplification conditions provided by Scientist

Primers, if required, are charged extra

In case PCR fails (upon repeated attempts), we charge 

SEQ 09

 

 

 

 

 

SEQ 09F

 HLA Class I Typing

HLA-A/B/C typing by sequencing 

Exon 2 and 3 amplified

Genomic DNA isolation included

Sequence data provided

HLAC 1

HLA Class II Typing

HLA- DRB1/ 3/ 4/ 5/ DBP1/ DQA1/ DQB1 typing by sequencing 

Exon 2 and 3 amplified

Genomic DNA isolation included

Sequence data provided

HLAC 2

SSCP analysis

 

SSCP 01

PCR, Cloning & Sequencing

500 bp or smaller amplicons, 1- 4 samples

500 bp or smaller amplicons, >4 samples

500 bp to 1.0 kb amplicons, 1- 4 samples

500 bp to 1.0 kb amplicons, >4 samples

1.0 kb to 2.0 kb amplicons, 1- 4 samples

1.0 kb to 2.0 kb amplicons, >4 samples

CSS 01

CSS 02

CSS 03

CSS 04

CSS 05

CSS 06

Contact services@chromous.com or call @ 09448605734 for more details

Careers

Marketing Executive: Chennai/Kerala/Bangalore

Fresh M.Sc/B.Tech in life science or 1 Year experience in marketing

Scientist: Bangalore

1-2 years experience in molecular Biology/ PhD in Life Science

Send your CV to vandana@chromous.com