Gene Silencing by Lentivirus based protocols
shRNA lentiviral services:
- We design shRNA sequences for the genes of your interest (or use your validated shRNAs
- We construct the lentiviral inducible shRNA expression clones and generate 0.5ml of Ready-to-use shRNA lentivirus particles (1x107 IFU/ml)
- We also provide you the free negative control shRNA lentiviral particles with corresponding selected marker
Over-Expression lentiviral services:
- We construct lentiviral expression clones for your target genes and generate 0.5 ml of ready-to-use high-titer expression lentivirus.
- You have the options with different promoters (suCMV, inducible CMV, EF1a or CAG) and different selection markers or make fluorescent-target fusion, and 6His-tag at N-term or C-term of your target is optional.
miRNA expression and anti-miRNA lentivirus services:
- miRNA expression: Construct expression lentivector and produce ready-to-use lentivirus for the precursor microRNA expression for you selected human or mouse miRNA listed in miRBase databse
- Anit-miRNA: Construct anti-miRNA lentivector and produce ready-to-use lentivirus for inhibit any miRNA listed in miRBase database.
Virus Production (packaging) service:
- We also provide service for lentivirus production. You just provide us your lentiviral expression vector; we deliver to you the pre-made, ready-to-use virus with the best titer possible from your vector.
Gene Knock-out / Knock-in and Gene Silencing Services
Knock-out / Knock-in of specific gene(s)
- A gene or set of genes can be knocked-out or knocked-in based on this protocol
- The targeted gene(s) are PCR amplified, cloned and sequence confirmed
- The knock-in / knock-out constructs are designed, developed and sequence confirmed
- The Gene / gene-set is inserted within the host genome within target region by site-specific homologous recombination protocol
- The insertion / deletion of foreign gene(s) would be sequence-specific / site-specific
siRNA Design and Synthesis
- Designed and synthesized for different systems
- Size: ~250-500 bp dsRNA, 3 nos. of dsRNA per target gene are prepared, 50 µg each
- siRNA and shRNA are synthesized, site-less cloning
- performed for perfect transcript production, no extra bases
Drosophila siRNA
- Available for silencing all drosophila genes individually
- Gene-specific, provided 10 µg enough for >10 experiments
Contact services@chromous.com or call @ 09448605734 for more details
