Restriction Endonucleases

Cutting Site

Buffer

Catalogue No.

Pack Size

Acc I

GT*MKAC

CB 4

REN 001

500 units

Apa I

GGGCC*C

CB 4 + BSA

REN 002A

500 units

Ava I

C*YCGRG

CB 4

REN 003A

500 units

BamH I

G*GATCC

CB 3 + BSA

REN 004A

2000 units

Bcl I

T*GATCA

CB 3

REN 005A

500 units

Bgl I

GCCNNNN*NGGC

CB 3

REN 006A

500 units

Bgl II

A*GATCT

CB 3

REN 007A

500 units

Cla I

AT*CGAT

CB 4 + BSA

REN 008A

500 units

EcoR I

G*AATTC

CB 1

REN 009A

5000 units

EcoR V

GAT*ATC

CB 3 + BSA

REN 010

500 units

Hind III

A*AGCTT

CB 2

REN 011A

5000 units

Hinf I

G*ANTC

CB 4

REN 012A

200 units

Hpa I

GTT*AAC

CB 4

REN 013 A

500 units

Hpa II

C*CGG

CB 1

REN 014A

500 units

Kpn I

GGTAC*C

CB 1

REN 015A

1000 units

Mbo I

*GATC

CB 4

REN 016

500 units

NdeI

CA*TATG

CB 1

REN 017A

500 units

Nco I

C*CATGG

CB 3

REN 018

500 units

Not I

GC*GGCCGC

CB 3 + BSA

REN 019

500 units

Nru I

TCG*CGA

CB 3

REN 020

500 units

Pst I

CTGCA*G

CB 3 + BSA

REN 021A

2000 units

Pvu II

CAG*CTG

CB 2

REN 022A

500 units

Sal I

G*TCGAC

CB 3 + BSA

REN 023A

500 units

Sau96 I

G*GNCC

CB 4

REN 024

500 units

Sfi I

GGCCNNNN*NGGCC

CB 4 + BSA

REN 025

500 units

Sma I

CCC*GGG

CB 4

REN 026A

500 units

Spe I

A*CTAGT

CB 4 + BSA

REN 027 A

200 units

Ssp I

AAT*ATT

CB 1

REN 028

200 units

Stu I

AGG*CCT

CB 4

REN 029

200 units

Taq I

T*CGA

CB 4 + BSA

REN 030A

500 units

Xba I

T*CTAGA

CB 4 + BSA

REN 031

500 units

Xho I

C*TCGAG

CB 4 + BSA

REN 032

500 units

Xma I

C*CCGGG

CB 4 + BSA

REN 033A

200 units

Xmn I

GAANN*NNTTC

CB 4 + BSA

REN 034A

500 units

Modifying Enzyme

Specifications

Catalogue No.

Pack Size

Terminal Transferase

Extends 3'-OH terminus

Template independent polymerase

MFE 01

100 Units

Agarase

Cleaves agarose subunits

Agarose gel pieces can be digested and DNA fragments eluted

MFE 02

50 Units

T4 Polynucleotide
Kinase

Catalyzes transfer and exchange of inorganic phosphate at 3' end of DNA

MFE 03

250 Units

Alkaline Phosphatase (Calf Intestine Phosphatase)

Catalyzes removal of 5' phosphate group

Obtained from Calf Intestinal mucosa

MFE 04

200 Units

Uracil DNA Glycosylase

Catalyzes release of free Uracil

MFE 05

200 Units

T4 DNA Ligase

Ligates blunt/ cohesive ends of DNA

Repairs single stranded nicks in duplex DNA/ RNA or hybrid

Tested for absence of Nucleases

MFE 06

MFE 06 S

1,00,000 Units

50,000 Units

DNA Polymerase I (klenow)

DNA polymerization  at 5'  to 3' direction

Has 3' to 5' exonuclease activit y

MFE 07

100 Units

T7 RNA Polymerase

Used for in vitro synthesis of RNA from single stranded or double stranded DNA

MFE 08

1000 Units

AMV Reverse
Transcriptase

RNA directed DNA polymerase

Synthesizes first strand cDNA

Can reverse transcribe long RNAs (up to 10 kb)

Success of reaction heavily depends on RNA quality

MFE 09

100 Units

M-MuLV Reverse Transcriptase

RNA directed DNA polymerase,

Native enzyme, has RNase H activity

Synthesizes first strand cDNA

Can reverse transcribe RNAs upto 3 kb long

Success of reaction heavily depends on RNA quality

MFE 10

1000 Units

M-MuLV Reverse Transcriptase (RNase H Negative)

RNA directed DNA polymerase

Genetically modified, RNase H negative

Synthesizes first strand cDNA

Can reverse transcribe RNAs upto 7 kb long

Success of reaction heavily depends on RNA quality

MFE 10A

1000 Units

DNAseI (RNase Free)

Endonuclease that cleaves DNA nonspecifically

MFE 11

1000 Units

RNase H

Endoribonuclease

Degrades phosphodiester bonds of RNA- DNA hybrids during first strand cDNA synthesis

MFE 12

100 Units

Ribonuclease A (DNase Free)

Powder; Molecular Biology Grade

MFE 13

MFE 13 S

500 mg

100 mg

Quick Ligase

Ligates blunt / cohesive ends of DNA

5 mins Ligation

Works the best with E. coli CB5α Competent cells 

MFE 14

100 Ligations