PCR Reagents
PCR Enzymes and Kits: FastPCRTM
FastPCRTM Reagents: Perform routine and complex PCRs in flat 40-50 min.
Get 4 to 5 FastPCRTM runs in the time of one Standard PCR run
Fast Q-PCR Reagents: Perform all routine and complex Q-PCRs in Fast and Standard formats
|
Product |
Specifications |
Catalogue No. |
PackSize |
|
FasTaq™ Polymerase |
Polymerizes @ 500 bp/ sec Just 1 sec extension for 500 bp amplicon Just 2 sec extension for 1 kb amplico FastPCRTM cycle condition: 500 bp
FastPCRTM cycle condition: 1 kb
Fidelity similar to Taq Polymerase The enzyme works in all standard PCR Machines Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc. PCR Controls (template and primers) provided |
FCN 01 |
300 Units |
|
FasTaq HiFi™ Polymerase |
Fidelity similar to Pfu Polymerase Polymerizes @ 500 bp/ sec Just 1 sec extension for 500 bp amplicon Just 2 sec extension for 1 kb amplicon FastPCRTM cycle condition: 500 bp
FastPCRTM cycle condition: 1 kb
The enzyme works in all standard PCR Machines Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc. PCR Controls (template and primers) provided.
|
FCN 02
|
300 Units
|
|
FastLong™ Polymerase |
FasTaq and FasTaq HiFi Enzyme mix for better fidelity High Fidelity: 13x10-6 Polymerizes @ 500 bp per sec. Just 30 sec extension for 5 kb amplicon FastPCRTM cycle condition: 5 kb
The enzyme works in all standard PCR Machines Amplifies upto 5 kb PCR Controls provided |
FCN 03
|
300 Units
|
|
FastPCR™ Master -Mix |
FasTaqTM Polymerase –based kit FastPCRTM Cycle conditions: 40 min for 30 cycle PCR All PCR components added at 2X concentration, just add template, primer and water to start FastPCRTM |
FCN 04
|
5 x 1 ml
|
Standard PCR
|
Product |
Specifications |
Catalogue No. |
Pack Size |
|
Taq DNA Polymerase |
3U/ µl, buffer with MgCl2, PCR Controls provided |
PCN 01 |
300 Units |
|
3U/ µl, buffer with MgCl2, PCR Controls provided |
PCN 02 |
2 x 300 Units |
|
|
3U/ µl, buffer with MgCl2, PCR Controls provided |
PCN 03 |
5x 300 Units |
|
|
1U/ µl, buffer with MgCl2, PCR Controls provided |
PCN 07 |
2 x 300 Units |
|
|
1U/ µl, MgCl2 provided separately, PCR Controls provided |
PCN 08 |
2 x 300 Units |
|
|
1U/ µl, MgCl2 provided separately, PCR Controls provided |
PCN 09 |
5 x 300 Units |
|
|
3U/ µl, MgCl2 provided separately, PCR Controls provided |
PCN 04 |
300 Units |
|
| 3U/ µl, MgCl2 provided separately, PCR Controls provided | PCN 05 | 2 x 300 Units | |
| 3U/ µl, MgCl2 provided separately, PCR Controls provided | PCN 06 | 5x 300 Units | |
|
GreenTaq™ Polymerase |
Green colored Taq for easy viewing and loading 3U/ µl, Buffer contains MgCl2 PCR positive controls provided with every purchase |
PCN 13
|
300 Units |
|
Green colored Taq for easy viewing and loading 3U/ µl, MgCl2 provided seperatly PCR Controls provided with every purchase |
PCN 14
|
300 Units
|
|
|
Fidelity Polymerase |
Highest known Fidelity: 1.5 x 10-6 (1.5 mutations in a million bases incorporated Similar to Pfu Polymerase PCR positive Controls provided with every purchase |
PCN 10
|
300 Units
|
|
ChromTaq™ Polymerase |
Enzyme mix of Taq polymerase and Fidelity polymerase High Fidelity: 13 x 10-6 Can be used for all regular PCRs Amplifies up to 5 kb PCR positive Controls provided with every purchase |
PCN 11
|
300 Units
|
|
XTRA™ Polymerase |
For 3-20 kb amplification For usage with AutoExcel PCR Protocol PCR positive controls provided with every purchase |
PCN 12 |
300 Units |
| PCR Master- Mix (2 X) |
Taq Polymerase-based kit 2X-mix (make up volume with template, primers and water) |
PCN 24 | 5 x 1 ml |
|
ChromTaq Master-Mix |
ChromTaq-based high fidelity PCR kit, provided as 2X 2X-mix (make up volume with template, primers and water)
|
PCN 17 |
5 x 1 ml |
|
GC-PCR Solution |
Good for GC-rich repeat amplification (Fragile-X) Good for amplification of GC-rich genes (genes from M. tuberculosisgenome)
|
PCN 18 |
1 ml |
| Long-PCR Master-Mix | 5-20 kb amplification kit for plasmids/ genomes | PCN 20 | 5 x 1 ml |
| GreenTaq Master Mix |
GreenTaq™ Polymerase-based kit 2X-mix (make up to volume with template, primer and water) |
PCN 19 | 5 x 1 ml |
| RedTaq polymerase |
Red colored Taq for easy viewing and loading 3U/ µl, Buffer contains MgCl2 PCR positive controls provided with every purchase Red colored Taq for easy viewing and loading |
PCN 15 | 300 Units |
| RedTaq polymerase |
Red colored Taq for easy viewing and loading 3U/ µl, MgCl2 provided separately PCR positive controls provided with every purchase Red colored Taq for easy viewing and loading |
PCN 16 | 300 Units |
| RedTaq Master-Mix |
Red Taq Polymerase -based kit 2X -mix (make up volume with template, primers and water) |
PCN 25 | 5 x 1 ml |
| AMV Reverse Transcriptase |
RNA directed DNA polymerase, synthesizes first strand cDNA Can reverse transcribe long RNAs (up to 10 kb) Success of reaction heavily depends on RNA quality |
MFE 09 | 100 Units |
| M-MuLV Reverse Transcriptase |
RNA directed DNA polymerase Native enzyme, has RNaseH activity Synthesizes first strand cDNA Can reverse transcribe RNAs upto 3 kb long Success of reaction heavily depends on RNA quality |
MFE 10 | 1000 Units |
|
M-MuLV Reverse Transcriptase (RNase H Negative) |
RNA directed DNA polymerase Genetically modified, RNase H negative Synthesizes first strand cDNA Can reverse transcribe RNAs up to 7 kb long Success of reaction heavily depends on RNA quality |
MFE 10 A | 1000 Units |
| RT-PCR Kit (M-MuLV based) |
For mRNA/ cDNA of ~3.0 kb Robust amplification 5 control reactions |
PCN 28 | 10 Reactions |
|
RT-PCR Kit (M-MuLV, Single tube) |
For mRNA/ cDNA of ~3.0 kb RT and PCR are performed in same tube Robust amplification 3 control reactions |
PCN 29 | 10 Reactions |
| RT-PCR Kit (AMV) |
For mRNA / cDNA of >3.0 kb Robust amplification 5 control reactions |
PCN 26 | 10 Reactions |
| RT-PCR Kit (AMV, Single tube) |
For mRNA/ cDNA of >3.0 kb RT and PCR in same tube Robust amplification 3 control reactions |
PCN 27 | 10 Reactions |