PCR Reagents

PCR Enzymes and Kits: FastPCRTM

FastPCRTM Reagents: Perform routine and complex PCRs in flat 40-50 min.

Get 4 to 5 FastPCRTM runs in the time of one Standard PCR run

Fast Q-PCR Reagents: Perform all routine and complex Q-PCRs in Fast and Standard formats

Product

Specifications

Catalogue No.

PackSize

FasTaq™ Polymerase

Polymerizes @ 500 bp/ sec

Just 1 sec extension for 500 bp amplicon

Just 2 sec extension for 1 kb amplico

FastPCRTM cycle condition: 500 bp

FastPCRTM cycle condition: 1 kb

Fidelity similar to Taq Polymerase

The enzyme works in all standard PCR Machines

Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc.

PCR Controls (template and primers) provided

FCN 01

300 Units

   

FasTaq HiFi™ Polymerase

Fidelity similar to Pfu Polymerase

Polymerizes @ 500 bp/ sec

Just 1 sec extension for 500 bp amplicon

Just 2 sec extension for 1 kb amplicon

FastPCRTM cycle condition: 500 bp

FastPCRTM cycle condition: 1 kb

The enzyme works in all standard PCR  Machines

Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc.

PCR Controls (template and primers) provided.

 

FCN 02

 

 

300 Units

 

 

FastLong™ Polymerase

FasTaq and FasTaq HiFi Enzyme mix for better fidelity

High Fidelity: 13x10-6

Polymerizes @ 500 bp per sec.

Just 30 sec extension for 5 kb amplicon

FastPCRTM cycle condition: 5 kb

The enzyme works in all standard PCR Machines

Amplifies upto 5 kb

PCR Controls provided

FCN 03

 

300 Units

 

FastPCR™ Master -Mix

FasTaqTM Polymerase –based kit

FastPCRTM Cycle conditions: 40 min for 30 cycle PCR

All PCR components added at 2X concentration, just add template,

primer and water to start FastPCRTM 

FCN 04

 

5 x 1 ml

 

 Standard PCR

Product

Specifications

Catalogue No.

Pack Size

 

 

 

 Taq DNA Polymerase

3U/ µl, buffer with MgCl2, PCR Controls provided

PCN 01

300 Units

3U/ µl, buffer with MgCl2, PCR Controls provided

PCN 02

2 x 300 Units 

3U/ µl, buffer with MgCl2, PCR Controls provided

PCN 03

5x 300 Units

1U/ µl, buffer with MgCl2, PCR Controls provided

PCN 07

2 x 300 Units 

1U/ µl, MgClprovided separately, PCR Controls provided

PCN 08

2 x 300 Units 

1U/ µl, MgCl2 provided separately, PCR Controls provided

PCN 09

5 x 300 Units 

3U/ µl, MgClprovided separately, PCR Controls provided

PCN 04

300 Units

3U/ µl, MgClprovided separately, PCR Controls provided PCN 05 2 x 300 Units 
3U/ µl, MgClprovided separately, PCR Controls provided PCN 06 5x 300 Units

 

 

GreenTaq™ Polymerase

Green colored Taq for easy viewing and loading

3U/ µl, Buffer contains MgCl2 

PCR positive controls provided with every purchase   

PCN 13

     

300 Units

Green colored Taq for easy viewing and loading

3U/ µl, MgCl2 provided seperatly                     

PCR Controls provided with every purchase

PCN 14

 

300 Units

 

Fidelity Polymerase

Highest known Fidelity: 1.5 x 10-6 (1.5 mutations in a million

bases incorporated

Similar to Pfu Polymerase

PCR positive Controls provided with every purchase

PCN 10    

 

300 Units 

 

ChromTaq™ Polymerase

Enzyme mix of Taq polymerase and Fidelity polymerase

High Fidelity: 13 x 10-6

Can be used for all regular PCRs

Amplifies up to 5 kb

PCR positive Controls provided with every purchase

PCN 11

 

300 Units

 

XTRA™ Polymerase

For 3-20 kb amplification

For usage with AutoExcel PCR Protocol

PCR positive controls provided with every purchase

PCN 12

300 Units

PCR Master- Mix (2 X)

Taq Polymerase-based kit

2X-mix (make up volume with template, primers and water)

PCN 24  5 x 1 ml

ChromTaq Master-Mix

 

ChromTaq-based high fidelity PCR kit, provided as 2X

2X-mix (make up volume with template, primers and water)

 

PCN 17

5 x 1 ml

GC-PCR Solution

 

Good for GC-rich repeat amplification (Fragile-X)

Good for amplification of GC-rich genes

(genes from M. tuberculosisgenome)

 

PCN 18

1 ml

Long-PCR Master-Mix 5-20 kb amplification kit for plasmids/ genomes PCN 20 5 x 1 ml
GreenTaq Master Mix

GreenTaq™  Polymerase-based kit

2X-mix (make up to volume with template, primer and water)

PCN 19 5 x 1 ml
RedTaq polymerase

Red colored Taq for easy viewing and loading

3U/ µl, Buffer contains MgCl2

PCR positive controls provided with every purchase

Red colored Taq for easy viewing and loading

PCN 15 300 Units
RedTaq polymerase

Red colored Taq for easy viewing and loading

3U/ µl, MgCl2 provided separately

PCR positive controls provided with every purchase

Red colored Taq for easy viewing and loading

PCN 16 300 Units
 RedTaq Master-Mix

Red Taq Polymerase -based kit

2X -mix (make up volume with template, primers and water)

PCN 25  5 x 1 ml
AMV Reverse Transcriptase

RNA directed DNA polymerase, synthesizes first strand cDNA 

Can reverse transcribe long RNAs (up to 10 kb)

Success of reaction heavily depends on RNA quality

MFE 09 100 Units
M-MuLV Reverse Transcriptase

RNA directed DNA polymerase

Native enzyme, has RNaseH activity

Synthesizes first strand cDNA

Can reverse transcribe RNAs upto 3 kb long

Success of reaction heavily depends on RNA quality

MFE 10 1000 Units

M-MuLV Reverse Transcriptase

(RNase H Negative)

RNA directed DNA polymerase

Genetically modified, RNase H negative

Synthesizes first strand cDNA

Can reverse transcribe RNAs up to 7 kb long

Success of reaction heavily depends on RNA quality

MFE 10 A 1000 Units
RT-PCR Kit (M-MuLV based)

For mRNA/ cDNA of ~3.0 kb

Robust amplification

5 control reactions

PCN 28 10 Reactions

RT-PCR Kit (M-MuLV,

Single tube)

For mRNA/ cDNA of ~3.0 kb

RT and PCR are performed in same tube

Robust amplification

3 control reactions

PCN 29 10 Reactions
RT-PCR Kit (AMV)

For mRNA / cDNA of >3.0 kb

Robust amplification

5 control reactions

PCN 26 10 Reactions
RT-PCR Kit (AMV, Single tube)

For mRNA/ cDNA of >3.0 kb

RT and PCR in same tube

Robust amplification

3 control reactions

PCN 27 10 Reactions

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