Gene Cloning Research Kits

 

Product

Specifications

Catalogue No.

Pack Size

Ready-to- Ligate Cloning Vectors

Single or double restricted as per requirement                                                                                                                    

Calf Intestinal Phosphatase treated

Checked for no / low self-ligation

PCN 34

20 Ligations

PCR Cloning Kit (T/A)

Cloning of PCR products, 10 reactions

Unpurified PCR products preferred for
Cloning

Cloning efficiency varies for amplicons

Guaranteed clones with E. coli CB5a Competent cells 

PCN 23

10 Reactions

Ligation Kit: Quick protocol

5 mins Ligation

Positive Controls (ready-to-ligate Vector & Insert) provided

Works best with E. coli CB5a Competent cells 

RKN 63

 

RKN 64

50 Ligations

 

100 Ligations

Competent Cells

E. coli (CB5 a)

Transformation efficiency: 5x106

Competent cells are send ready-to-use, can be stored at -70ºC for

2 weeks only, can not be stored at -20ºC

Please use 5-10 µl of ligation mix for best efficiency

The Competent cells are shipped on Dry-Ice

PCN 30

 

 

PCN 31

20 X 50 μl

 

 

10 X 50 µl

Cloning Kit (T/A) with Competent cells

T/A Cloning kit for 10 cloning reactions

10 vials of 50 µl competent cells

Competent cells are sent ready-to-use, can be stored at -70ºC for 2 weeks only, can not be stored at -20ºC

Shipped on Dry-Ice.

PCN 32

10 Clonings

cDNA First-Strand
Synthesis Kit

Starts from purified total RNA or mRNA

Oligo-dT or Random hexamer based synthesis using M-MuLV
Robust RNAseH negative M-MuLV RT provided

RKN 65

20 Reactions

cDNA Library Kit

Starts from purified total RNA or mRNA

Oligo-dT or Random hexamer based synthesis using M-MuLV
Reverse RNase H negative M-MuLV RT provided

Robust RNase H negative M-MuLV RT provided

Adapter ligation

PCR based library amplification / Normalization

Cloning of amplicons/ Library in “Ready-to-ligate” vectors

RKN 66

10 Reactions

DNA Labeling Kit: Random Priming

Random hexamer based

Can be adapted for radio-active or non- radioactive protocols

All Enzyme and reagents provided for 25 reactions

RKN 67

25 Reactions

DNA Labeling Kit: Nick Translation

DNase-1 created Nicks polymerized using DNA polymerase 1

Can be adapted for radio-active or non- radioactive protocols

All Enzyme and reagents provided for 25 reactions

RKN 68

25 Reactions

Competent Cell preparation kit

All Reagents for preparing 50 vials of 100 µl (5 ml) competent cells

Transformation efficiency of 5 x 106 efficiency achievable

Competent cells, once made, can be stored at -70ºC for 2 weeks only, can not be stored at -20ºC

Please use 5-10 µl of ligation mix for best efficiency

PCN 33

50 X100 μl

Silver Staining Kit: DNA 

Detects ~10 ng of DNA

25 min. protocol

High sensitivity

Kits for 10 and 25 gel staining

RKN 69

 

RKN 70

10 Gels

 

25 Gels

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Fresh M.Sc/B.Tech in life science or 1 Year experience in marketing

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1-2 years experience in molecular Biology/ PhD in Life Science

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