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Sequencing Requisition Form

Sample Requirements & Instructions:

Please indicate whether the sample is Purified or Unpurified
Purified Plasmids:

  • 1 or 2mg  (for every 500-600 bp read) , either in dried or in 10/20ml of Tris Hcl,pH 8.0-9.0.
  • Minimum template conc. should be 100 ng/ml and minimum volume should be 10ml.
  • Provide 0.5mg of more DNA for every additional reactions.

Unpurified Plasmids:

  • Please provide 4 or 5mg, either in dried or in Tris Hcl,pH 8.0-9.0.

 Purified PCR Products:

  • 30 ng DNA per 100bp product length , either in dried or in 20ml Tris-Cl, pH 8.0.
  • Minimum template conc. should be 100 ng/ml and minimum volume should be 10ml.
  • Provide 20ng more DNA per 100 bp product length for every additional reactions.

Unpurified PCR Products:

  • Please provide 4 or 5mg  , either in dried or in Tris Hcl,pH 8.0-9.0.

Primer:

  • Primer conc. shoud be 10pmol/ml, and minimum volume of 10 ml.
  • Provide 5ml of more primer for every additional reactions.
  • Enclose the sequence of your  primers in the provided column

Plasmid prepration:

  • Provide E. coli colonies on plate or pure stab culture in presence of antibiotic as selection marker.
  • Provide vector name, size, copy number and antibiotic name as a selection marker.

Special Instructions:

  • Please submit samples in  1.5ml micro centrifuge tubes.
  • Please do not use Tris EDTA buffer for eluting/dissolving your samples.
  • Please indicate if your samples have high GC content, repeats… in the special instruction column provided

Sample Collection: Tuesday & Friday (in every week)
Please Call: 011-41751641 or mail: delhi.chromous@gmail.com

Primer Details
Sl No. Sequence (5’ - 3’) Provided (Yes/NO) Concentration (pmol/ml)
Sample Details
Sl No. Sample Type (Plasmid/PCR) Sample Name Vector Insert/ Product Size ( kb) Concentration of Plasmid/ PCR Product Primers To Be Used Cat. No.

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